2,222 research outputs found
Identification and characterization of the human ORC6 homolog
A new protein was cloned and identified as the sixth member of the human Origin Recognition Complex (ORC). The newly identified 30-kDa protein hsORC6 is 28% identical and 49% similar to ORC6p from Drosophila melanogaster, which is consistent with the identities and similarities found among the other ORC members reported in the two species. The human ORC6 gene is located on chromosome 16q12. ORC6 protein level did not change through the cell cycle. Like ORC1, ORC6 did not co-immunoprecipitate with other ORC subunits but was localized in the nucleus along with the other ORC subunits. Several cellular proteins co-immunoprecipitated with ORC6, including a 65-kDa protein that was hyperphosphorylated in G1 and dephosphorylated in mitosis. Therefore, unlike the tight stoichiometric association of six yeast ORC subunits in one holo-complex, only a small fraction of human ORC1 and ORC6 is likely to be associated with a subcomplex of ORC2, 3, 4 and 5 suggesting differences in the architecture and regulation of human ORC
Inherent Weight Normalization in Stochastic Neural Networks
Multiplicative stochasticity such as Dropout improves the robustness and
generalizability of deep neural networks. Here, we further demonstrate that
always-on multiplicative stochasticity combined with simple threshold neurons
are sufficient operations for deep neural networks. We call such models Neural
Sampling Machines (NSM). We find that the probability of activation of the NSM
exhibits a self-normalizing property that mirrors Weight Normalization, a
previously studied mechanism that fulfills many of the features of Batch
Normalization in an online fashion. The normalization of activities during
training speeds up convergence by preventing internal covariate shift caused by
changes in the input distribution. The always-on stochasticity of the NSM
confers the following advantages: the network is identical in the inference and
learning phases, making the NSM suitable for online learning, it can exploit
stochasticity inherent to a physical substrate such as analog non-volatile
memories for in-memory computing, and it is suitable for Monte Carlo sampling,
while requiring almost exclusively addition and comparison operations. We
demonstrate NSMs on standard classification benchmarks (MNIST and CIFAR) and
event-based classification benchmarks (N-MNIST and DVS Gestures). Our results
show that NSMs perform comparably or better than conventional artificial neural
networks with the same architecture
Non Gaussian information of heterogeneity in Soft Matter
Heterogeneity in dynamics in the form of non-Gaussian molecular displacement
distributions appears ubiquitously in soft matter. We address the
quantification of such heterogeneity using an information-theoretic measure of
the distance between the actual displacement distribution and its nearest
Gaussian estimation. We explore the usefulness of this measure in two generic
scenarios of random walkers in heterogeneous media. We show that our proposed
measure leads to a better quantification of non-Gaussianity than the
conventional ones based on moment ratios
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CNS-Derived Blood Exosomes as a Promising Source of Biomarkers: Opportunities and Challenges.
Eukaryotic cells release different types of extracellular vesicles (EVs) including exosomes, ectosomes, and microvesicles. Exosomes are nanovesicles, 30-200 nm in diameter, that carry cell- and cell-state-specific cargo of proteins, lipids, and nucleic acids, including mRNA and miRNA. Recent studies have shown that central nervous system (CNS)-derived exosomes may carry amyloidogenic proteins and facilitate their cell-to-cell transfer, thus playing a critical role in the progression of neurodegenerative diseases, such as tauopathies and synucleinopathies. CNS-derived exosomes also have been shown to cross the blood-brain-barrier into the bloodstream and therefore have drawn substantial attention as a source of biomarkers for various neurodegenerative diseases as they can be isolated via a minimally invasive blood draw and report on the biochemical status of the CNS. However, although isolating specific brain-cell-derived exosomes from the blood is theoretically simple and the approach has great promise, practical details are of crucial importance and may compromise the reproducibility and utility of this approach, especially when different laboratories use different protocols. In this review we discuss the role of exosomes in neurodegenerative diseases, the usefulness of CNS-derived blood exosomes as a source of biomarkers for these diseases, and practical challenges associated with the methodology of CNS-derived blood exosomes and subsequent biomarker analysis
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